Antibacterial , fungicidal activity
Mycobacterium tuberculosis (Mtb) in vivo, in vitro

Result, opinion:
-The survival rate of mice administered the therapeutic dry birch bark extract (BED) the next day after infection Mtb was significantly higher than of those in control group.
-Inoculation of Mtb from animals' organs who got BED three weeks after being infected with a lethal dose of Mtb was  significantly lower than from animal organs in control group.
-The tested drugs in the therapeutic administration of BED showed antimycobacterial effect.
-Co-administration of BED with anti-TB drugs (ATD) significantly prolongs the survival of mice infected with Mtb compared to the control group of animals.
-Antimycobacterial BED effect directly to the mycobacteriain phagocytized by macrophages in in vitro system  is shown .
-The introduction of BED scheme into the standard specific therapy has showed a pronounced positive effect on repair processes in lungs, liver and spleen of experimental animals in comparison with the control group .
-Prophylactic administration of BED revealed:
-activation of immune cells (macrophages, monocytes and lymphocytes)
-the degree of activation of immune cells when BED administered is dose-dependent
-increase of survival periods of test animals compared to those in control group.

“Birch World” Ltd.
“The report on pilot studies assessing the effectiveness of  dry birch bark extract containing betulinol not less than 70 % and 98 %”
“The report on pilot studies assessing the effectiveness of  dry birch barkextract containing betulinol not less than 70 %”
“The report on pilot studies assessing the effectiveness of  dry birch barkextract containing betulinol not less than 70 % and 98 % when administered prophylactically”
Under the leadership of Director of State University CRIT RAMS, corresponding member of RAMS, professor V. Erokhina

In-vitro, 18-hour agar culture of microorganisms Staphylococcus aureus, Bacillus antracoides, Bacillus pyocyaneum, Escherichia coli, Solmonella typhimurium, Shigella Flexneri, Proteus vulgaris, strains of pathogenic dermatophyte fungi Candida albicans, Aspergillus nigerugr.

Result, opinion:  
The minimum inhibitory concentration (mcg/ml)  of triterpenoid drug was 160 mcg/ml for  Staphylococcus aureus, 200  mcg/ml for Bacillus antracoides, Escherichia coli и Solmonella typhimurium. The amount of triterpenoids from birch bark appeared to be effective against Aspergillus niger [80 mcg/ml], and to a lesser extent against Candida albicans. [125 mcg/ml]. Against the rest of fungi the activity of the amount of birch bark triterpenoids was not revealed.
It can be concluded that the sum of triterpenoids from birch bark is an effective bacteriostatic agent against microorganisms such as Staphylococcus aureus, Bacillus antracoides, Eschericha coli and Salmonella typhimurium, and also retards the growth of dermatophyte fungi Candida albicans and Aspergillus niger. High activity against Aspergillus niger in prospective opens  possibilities for the use of birch triterpenoids in veterinary with regard to frequent contamination of farm animals with these microorganisms.

Chapter 7.
The study of biological activity of triterpenoids from birch bark and roots of false licorice.

The thesis for the degree of Doctor of Pharmacy V. Semchenko, Pyatigorsk, 1993.

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